Molecular Analysis of Genetic Diversity: Advantages and Limitations
نویسندگان
چکیده
Abstract—Genetic diversity in plant materials used for revegetation of disturbed sites is important to allow natural selection and adaptation to occur. Several methods for estimation of genetic diversity using molecular markers are available. Protein markers, such as isozymes and seed storage proteins, have been used extensively, but their use has declined in recent years in favor of DNA markers. Use of protein markers is limited by lack of polymorphisms, number of markers available, and the need to use different detection methods with isozymes. DNA markers, including RFLP and RAPD analyses, are reliable methods that are widely used. The major limitation is the cost of labor and supplies.
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